Recombinant PGP9.5 Antibody
This mAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1) . Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson's disease.
Product Specifications
CAS Number
9000-83-3
Specifications
Western blot: 1-2 µg/mL, Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P09936
Host
Mouse
Reactivity
Human, Rat
Immunogen
Recombinant human protein was used as the immunogen for the recombinant PGP9.5 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RUCHL1/775
Type
Recombinant
Applications
WB, FACS, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the recombinant PGP9.5 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant PGP9.5 antibody is available for research use only.
Storage Conditions
Store the recombinant PGP9.5 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic, ER membrane
Image Legend
Immunofluorescent staining of permeabilized human T98G cells with recombinant PGP9.5 antibody cocktail (green, clone rUCHL1/775) and Nucspot (red) .
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