Recombinant Biotin Antibody / Rabbit Monoclonal

This mAb recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Not Applicable

Host

Rabbit

Immunogen

Biotinylated sheep immunoglobulin was used as the immunogen for this recombinant Biotin antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

BTN/2032R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant Biotin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant Biotin antibody is available for research use only.

Storage Conditions

Store the recombinant Biotin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Target location

Image Legend

IHC: Formalin-fixed, paraffin-embedded human tonsil stained with biotinylated Kappa Light Chain Ab probe followed by anti-Biotin antibody (clone BTN/2032R) . No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.