Recombinant Neurofilament Antibody / Heavy

This MAb reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H) . Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H) . Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1-2 µg/mL, Flow cytometry: 0.5-1ug/10^6 cells, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

UniProt

P12036

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant human protein (heavy subunit) was used as the immunogen for the recombinant Neurofilament antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RNF421

Type

Recombinant

Applications

WB, FACS, IHC-P

Purity

Protein G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant Neurofilament antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant Neurofilament antibody is available for research use only.

Storage Conditions

Store the recombinant Neurofilament antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic, membranous

Image Legend

IHC testing of FFPE human brain with recombinant Neurofilament antibody (clone rNF421) . Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.