Recombinant MMP3 Antibody

The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB) . MMP catalysis requires both calcium and zinc. MMP3, MMP10 and MMP11 (also designated stromelysin-1, 2 and 3, respectively) activate procollagenase. MMP3 activation of procollagenase can occur via two pathways. Direct activation by MMP3 is slow and activation by MMP3 in conjunction with tissue or plasma proteinases is rapid. MMP10 is expressed in small intestine, and at lower levels in lung and heart. MMP11 is specifically expressed in stromal cells of breast carcinomas and contributes to epithelial cell malignancies.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P08254

Host

Mouse

Reactivity

Human

Immunogen

Amino acids 317-327 were used as the immunogen for the recombinant MMP3 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RMMP3/1730

Type

Recombinant

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant MMP3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant MMP3 antibody is available for research use only.

Storage Conditions

Store the recombinant MMP3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic, secreted

Image Legend

IHC testing of FFPE human placenta with recombinant MMP3 antibody (clone rMMP3/1730) . Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.