Recombinant CD146 Antibody

CD146 expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. It is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease.

Product Specifications

CAS Number

9000-83-3

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

P43121

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human protein was used as the immunogen for the recombinant CD146 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RMUC18/1130

Type

Recombinant

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the recombinant CD146 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant CD146 antibody is available for research use only.

Storage Conditions

Store the recombinant CD146 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Cell surface, cytoplasm

Image Legend

IHC testing of FFPE human tonsil with CD146 antibody (clone rMUC18/1130) . Required boiling tissue sections in 10mM Tris with 1mM EDTA, pH8, for 10-20 min followed by cooling at RT for 20 min.