Recombinant CD146 Antibody

CD146 expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. It is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

UniProt

P43121

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human protein was used as the immunogen for the recombinant CD146 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RMUC18/1130

Type

Recombinant

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant CD146 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant CD146 antibody is available for research use only.

Storage Conditions

Store the recombinant CD146 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cell surface, cytoplasm

Image Legend

IHC testing of FFPE human tonsil with CD146 antibody (clone rMUC18/1130) . Required boiling tissue sections in 10mM Tris with 1mM EDTA, pH8, for 10-20 min followed by cooling at RT for 20 min.