Recombinant Napsin A Antibody / Rabbit Monoclonal
Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B. There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL
UniProt
O96009
Host
Rabbit
Reactivity
Human
Immunogen
A recombinant fragment from the human protein (amino acids 189-299) was used as the immunogen for the recombinant Napsin A antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
NAPSA/1865R
Type
Recombinant
Applications
IHC-P, WB
Purity
Protein A affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the recombinant Napsin A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant Napsin A antibody is available for research use only.
Storage Conditions
Store the recombinant Napsin A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic
Image Legend
Western blot testing of human kidney tissue lysate with recombinant Napsin A antibody. Expected molecular weight: 38-45 kDa.
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