Recombinant CK10 Antibody / Rabbit Monoclonal

This mAb recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10) . CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 0.1-0.2 µg/mL for 30 min at RT

UniProt

P13645

Host

Rabbit

Reactivity

Human

Immunogen

A cytoskeletal preparation extracted from human ectocervical epithelium was used as the immunogen for the recombinant CK10 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

KRT10/1948R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the recombinant CK10 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant CK10 antibody is available for research use only.

Storage Conditions

Store the recombinant CK10 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human skin with recombinant CK10 antibody (clone KRT10/1948R) . Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.