Recombinant EpCAM Antibody / Cytoplasmic domain

EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM) . It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

UniProt

P16422

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 289-314 (cytoplasmic domain) was used as the immunogen for the recombinant EpCAM antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1

Clone

REGP40/1110

Type

Recombinant

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant EpCAM antibody is available for research use only.

Storage Conditions

Store the recombinant EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cell surface, cytoplasmic

Image Legend

IHC testing of FFPE human ovarian carcinoma with recombinant EpCAM antibody (clone rEGP40/1110) . Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.