Recombinant CK19 Antibody / Cytokeratin 19
Recognizes a protein of 40kDa, identified as cytokeratin-19 (CK19), which is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.
Product Specifications
CAS Number
9000-83-3
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P08727
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human Cytokeratin 19 protein was used as the immunogen for the recombinant CK19 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RKRT19/800
Type
Recombinant
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the recombinant CK19 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant CK19 antibody is available for research use only.
Storage Conditions
Store the recombinant CK19 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic, cell membrane, secreted
Image Legend
Western blot testing of human HepG2 cell lysate using recombinant CK19 antibody (clone rKRT19/800) . Predicted molecular weight ~43 kDa.
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