Recombinant KRT10 Antibody / Cytokeratin 10

This mAb recognizes a protein of 56.5kDa, identified as cytokeratin 10 (KRT10) . KRT10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of KRT10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, KRT10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), KRT10 is detected very frequently. Expression is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 0.1-0.2 µg/mL for 30 min at RT

UniProt

P13645

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human Cytokeratin 10 protein was used as the immunogen for the recombinant KRT10 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RKRT10/844

Type

Recombinant

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant KRT10 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant KRT10 antibody is available for research use only.

Storage Conditions

Store the recombinant KRT10 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human skin with recombinant KRT10 antibody (clone rKRT10/844) . Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.