Recombinant Keratin 10 Antibody

This mAb recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10) . CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.

Product Specifications

CAS Number

9000-83-3

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

P13645

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human Cytokeratin 10 protein was used as the immunogen for the recombinant Keratin 10 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RKRT10/1275

Type

Recombinant

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the recombinant Keratin 10 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant Keratin 10 antibody is available for research use only.

Storage Conditions

Store the recombinant Keratin 10 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human skin with recombinant Keratin 10 antibody (clone rKRT10/1275) . Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.