Recombinant b-Catenin Antibody

The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs) . AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. [RefSeq]

Product Specifications

CAS Number

9000-83-3

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

P35222

Host

Rabbit

Reactivity

Human

Immunogen

Human protein was used as the immunogen for the recombinant b-Catenin antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

CTNNB1/2030R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the recombinant b-Catenin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant b-Catenin antibody is available for research use only.

Storage Conditions

Store the recombinant b-Catenin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Cell surface, cytoplasmic, cell junctions

Image Legend

IHC testing of FFPE human cervical carcinoma with recombinant b-Catenin antibody (clone CTNNB1/2030R) . Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.