Recombinant CD66 Antibody / CEA / CEACAM5

CEA are glycosyl phosphatidyl inositol (GPI) cell-surface-anchored glycoproteins whose specialized sialofucosylated glycoforms serve as functional colon carcinoma L-selectin and E-selectin ligands, which may be critical to the metastatic dissemination of colon carcinoma cells. Immunologically they are characterized as members of the CD66 cluster of differentiation. [Wiki]

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT (1) (2)

UniProt

P06731

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant human protein was used as the immunogen for this recombinant CD66 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

C66/2055R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant CD66 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant CD66 antibody is available for research use only.

Storage Conditions

Store the recombinant CD66 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic and luminal surface

Image Legend

IHC testing of FFPE human colon carcinoma with CD66 antibody (clone C66/2055R) . Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.