Recombinant RB1 Antibody / Rabbit Monoclonal
Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P06400
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A human recombinant partial protein was used as immunogen for this recombinant RB1 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
RB1/2313R
Type
Recombinant
Applications
IHC-P
Purity
Protein A affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
The recombinant RB1 antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.
Limitations
This recombinant RB1 antibody is available for research use only.
Storage Conditions
The recombinant RB1 antibody (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Nuclear
Image Legend
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
Human CKM (Creatine Kinase, Muscle) ELISA Kit
Human CKM (Creatine Kinase, Muscle) ELISA Kit
Human CKM (Creatine Kinase, Muscle) ELISA Kit
