Recombinant ANO1 Antibody / DOG1 / TMEM16A
Expression of DOG1/ANO1 protein is elevated in the gastrointestinal stromal tumors (GIST's), c-kit signaling-driven mesenchymal tumors of the GI tract. It is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for ANO1 has been reported in 97.8 percent of scorable GIST's, including all c-kit negative GIST's. Overexpression has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha mutants that fail to express c-kit antigen. The overall sensitivity of ANO1 and c-kit in GIST's is nearly identical: 94.4% vs. 94.7%.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
Q5XXA6
Host
Mouse
Reactivity
Human
Immunogen
A recombinant human full length protein was used as the immunogen for this recombinant ANO1 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RDG1/447
Type
Recombinant
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the recombinant ANO1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant ANO1 antibody is available for research use only.
Storage Conditions
Store the recombinant ANO1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cell Surface and Cytoplasmic
Image Legend
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
CD35 / CR1 (E11), CF488A conjugate, 0.1mg/mL
Ep-CAM / CD326 (Epithelial Marker) (rVU-1D9), CF594 conjugate, 0.1mg/mL
