Recombinant CK8 Antibody / Cytokeratin 8
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18) . Cytokeratin 8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibody that recognizes only cytokeratin 8 and 18. Cytokeratin 8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT, Western blot: 1-2 µg/mL
UniProt
P05787
Host
Mouse
Reactivity
Human
Immunogen
A cytoskeletal preparation from human HeLa cells was used as the immunogen for this recombinant CK8 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RB22.1
Type
Recombinant
Applications
IHC-P, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the recombinant CK8 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant CK8 antibody is available for research use only.
Storage Conditions
Store the recombinant CK8 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic
Image Legend
Western blot testing of human HCT-116 cell lysate with recombinant CK8 antibody (clone rB22.1) .
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