Recombinant Thrombomodulin Antibody / CD141

It recognizes a protein of 75kDa, identified as Thrombomodulin. Thrombomodulin is a transmembrane glycoprotein with natural anticoagulant properties. It is normally expressed by a restricted number of cells, such as endothelial and mesothelial cells. In addition, synovial lining and syncytio-trophoblasts of placenta also express thrombomodulin. This protein is present in almost all of benign vascular tumors and majority of malignant vascular tumors (Kaposi s sarcoma, angiosarcoma, and epithelioid hemangioendothelioma) . Hence, anti-thrombomodulin serves as a sensitive marker for lymphatic endothelial cells and their tumors. Recently, thrombomodulin antibody has been used for mesothelial cells and malignant mesotheliomas.

Product Specifications

CAS Number

9000-83-3

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

P07204

Host

Mouse

Reactivity

Human

Immunogen

Human recombinant partial protein corresponding to amino acids 69-194 was used as the immunogen for this recombinant Thrombomodulin antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RTHBD/1591

Type

Recombinant

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the recombinant Thrombomodulin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant Thrombomodulin antibody is available for research use only.

Storage Conditions

Store the recombinant Thrombomodulin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Cell surface, cytoplasmic

Image Legend

IHC testing of FFPE human cervical carcinoma with recombinant Thrombomodulin antibody (clone rTHBD/1591) . HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.