Recombinant CFTR Antibody

CFTR functions as an ATP-gated anion channel, increasing the conductance for certain anions (e.g. Cl− ) to flow down their electrochemical gradient. ATP-driven conformational changes in CFTR open and close a gate to allow transmembrane flow of anions down their electrochemical gradient.[5] This in contrast to other ABC proteins, in which ATP-driven conformational changes fuel uphill substrate transport across cellular membranes. Essentially, CFTR is an ion channel that evolved as a 'broken' ABC transporter that leaks when in open conformation. CFTR is found in the epithelial cells of many organs including the lung, liver, pancreas, digestive tract, and the reproductive tract. [Wiki]

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

UniProt

P13569

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human partial protein was used as the immunogen for this recombinant CFTR antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RCFTR/1342

Type

Recombinant

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant CFTR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant CFTR antibody is available for research use only.

Storage Conditions

Store the recombinant CFTR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cell surface, cytoplasmic

Image Legend

IHC testing of FFPE human pancreas with recombinant CFTR antibody (clone rCFTR/1342) . HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9 for 10-20 min followed by cooling at RT for 20 min.