Recombinant CD8A Antibody

CD8 is a cell surface receptor expressed either as a heterodimer with the beta chain or as a homodimer. A majority of thymocytes and a subpopulation of mature Tcells and NK cells express CD8a. It binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in Tcell development and activation of mature Tcells. For mature Tcells, CD8 and 4 are mutually exclusive, so antibody to CD8 is generally used in conjunction with antibody to CD4. CD8a antibody is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral Tcell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+/CD8-, and in T-lymphoblastic lymphoma/leukemia, they are often co-expressed. It is also found in littoral cell angioma of the spleen.

Product Specifications

CAS Number

9000-83-3

Specifications

ELISA: 2-4 µg/mL (order BSA/azide-free format), Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P01732

Host

Mouse

Reactivity

Human

Immunogen

Human recombinant protein was used as the immunogen for this recombinant CD8A antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RC8/468

Type

Recombinant

Applications

ELISA, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the recombinant CD8A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant CD8A antibody is available for research use only.

Storage Conditions

Store the recombinant CD8A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cell surface

Image Legend

IHC testing of FFPE human colon tissue with recombinant CD8A antibody (clone rC8/468) . Required HIER: boil tissue sections in pH9 EDTA, for 10-20 min followed by cooling at RT for 20 min.