TYMS Antibody / Thymidylate Synthase
This antibody recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS, TYMS) . TS converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P04818
Host
Mouse
Reactivity
Human
Immunogen
A recombinant human parital protein corresponding to amino acids 60-174 was used as the immunogen for this TYMS antibody.
Clonality
Monoclonal
Isotype
IgG2c κ
Clone
TYMS/1884
Applications
FACS, IF, WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the TYMS antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This TYMS antibody is available for research use only.
Storage Conditions
Store the TYMS antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Location
Cytoplasmic, nuclear
Image Legend
Immunofluorescent staining of PFA-fixed human Ramos cells with TYMS antibody cocktail (green, clone TYMS/1884) and Phalloidin (red) .
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