S100B Antibody

S100 proteins are localized in the cytoplasm and nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100B may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. In the developing CNS it acts as a neurotrophic factor and neuronal survival protein. In the adult organism it is usually elevated due to nervous system damage, which makes it a potential clinical marker. [Wiki]

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1-2 µg/mL, Immunohistology (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

UniProt

P04271

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human S100B protein was used as the immunogen for the S100B antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

PS1B1-1

Applications

IF, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the S100B antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This S100B antibody is available for research use only.

Storage Conditions

Store the S100B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the S100B antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Location

Cytoplasmic, nuclear

Image Legend

Confocal Immunofluorescent analysis of A2058 cells using Alexa Fluor 488-labeled S100B antibody (green) . F-actin filaments were labeled with DyLight 554 Phalloidin (red) . DAPI was used to stain the cell nuclei (blue) .