Creatine kinase B Antibody / CKB
Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type) . There are three different isoenzymes: CKMM, CKBB and CKMB. This mAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB. It shows minimal reactivity with other human serum proteins
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA (order BSA/sodium azide-free format for coating), Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL
UniProt
P12277
Host
Mouse
Reactivity
Human
Immunogen
Human CKB protein was used as the immunogen for the Creatine kinase B antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
2ba6
Applications
ELISA, FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Creatine kinase B antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Creatine kinase B antibody is available for research use only.
Storage Conditions
Store the Creatine kinase B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Creatine kinase B antibody should be determined by the researcher.
Location
Cytoplasmic
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