MUC16 Antibody

The mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) . Membrane-associated and secretory mucins are high molecular weight glycoproteins of the glycocalyx (polysaccharide biofilm) that protects mucosal epithelium from particulate matter and microorganisms. Epithelial mucins are large, secreted and cell surface glycoproteins crucial for adhesion modulation, signaling and epithelial cell protection. The number of repeats is highly polymorphic and varies among different alleles. The Mucin family consists of Mucins 1-4, Mucin 5 (AC and B), 6-8, 11-13 and 15-17. The Mucin 16 protein (also commonly referred to as CA125), encoded for by the gene MUC16, is a very high molecular weight tumor antigen consisting of three domains: a carboxy terminal domain, an extracellular domain and an amino terminal domain. Mucin 16, an ovarian cancer-associated antigen, is used as a marker to monitor the progress of epithelial ovarian cancer. It is a hydrophilic membrane-associated protein that may be involved in vitamin A functions.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q8WXI7

Host

Mouse

Reactivity

Human

Immunogen

An enzymatically glycosylated fragment of human MUC16 recombinant protein was used as the immunogen for the MUC16 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

5E11.

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the MUC16 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This MUC16 antibody is available for research use only.

Storage Conditions

Store the MUC16 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the MUC16 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Location

Cell surface, cytoplasmic, secreted

Image Legend

IHC testing of FFPE human ovarian carcinoma with MUC16 antibody (clone 5E11) . Required HIER: steam section in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool prior to testing. Data courtesy of Dr. Leonor David, IPATIMUP and Medical Faculty University of Porto, Portugal.