Recombinant Basic Cytokeratin Antibody / Rabbit Monoclonal

This mAb recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1) ; 64kDa (CK3) ; 59kDa (CK4) ; 58kDa (CK5) ; 56kDa (CK6) ; 52kDa (CK8) . Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL for 30 min at RT

UniProt

Q01546

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant human KRT76 protein was used as the immunogen for the recombinant Basic Cytokeratin antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

KRTH/1576R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant Basic Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant Basic Cytokeratin antibody is available for research use only.

Storage Conditions

Store the recombinant Basic Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Basic Cytokeratin antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human skin with recombinant Basic Cytokeratin antibody (clone KRTH/1576R) . Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.