CD81 Antibody

This mAb recognizes a protein of 26kDa, identified as CD81 (Workshop VI; Code CD81.1) . CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspans transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.

Product Specifications

CAS Number

9007-83-4

Specifications

Functional studies: order BSA/sodium azide-free format, Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 0.5-1 µg/mL, Western blot: 0.5-1 µg/mL for 2 hours at RT, Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 min at RT

UniProt

P60033

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full length human protein was used as the immunogen for the CD81 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

1.3.3.22

Applications

Functional studies, FACS, IF, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the CD81 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CD81 antibody is available for research use only.

Storage Conditions

Store the CD81 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the CD81 antibody should be determined by the researcher.

Location

Cytoplasmic, membranous

Image Legend

IHC testing of FFPE human tonsil with CD81 antibody (clone 1.3.3.22) . Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.