CD79a Antibody

A disulphide-linked heterodimer, consisting of mb-1 (or CD79a) and B29 (or CD79b) polypeptides, is non-covalently associated with membrane-bound immunoglobulins on B cells. This complex of mb-1 and B29 polypeptides and immunoglobulin constitute the B cell Ag receptor. CD79a first appears at pre B cell stage, early in maturation, and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. It is not present in myeloid or T cell lines.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL for 30 min at RT

UniProt

P11912

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 202-216 (GTYQDVGSLNIADVQ) were used as the immunogen for the CD79a antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the CD79a antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CD79a antibody is available for research use only.

Storage Conditions

Store the CD79a antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the CD79a antibody should be determined by the researcher.

Location

Cell surface, cytoplasmic

Image Legend

IHC testing of FFPE human tonsil with CD79a antibody. Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.