CD71 Antibody / Transferrin Receptor (Extracellular domain)

It recognizes a ~85-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa. This mAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: 2-4 µg/mL (order BSA/azide-free format), Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT Western blot: 0.5-1 µg/mL

UniProt

P02786

Host

Mouse

Reactivity

Human

Immunogen

Amino acids 94-212 (extracellular domain) of the human protein were used as the immunogen for the Transferrin Receptor / CD71 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

TFRC/1817

Applications

ELISA, IHC-P WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the CD71 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CD71 antibody is available for research use only.

Storage Conditions

Store the CD71 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the CD71 antibody should be determined by the researcher.

Location

Cell surface

Image Legend

IHC testing of FFPE human placenta tissue with Transferrin Receptor / CD71 antibody (clone TFRC/1817) . Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.