Recombinant EpCAM Antibody
Binding epitope of this antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59 of Ep-CAM. EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM) . It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Product Specifications
CAS Number
9000-83-3
Specifications
UniProt
P16422
Host
Mouse
Reactivity
Human
Immunogen
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RMOC-31
Type
Recombinant
Applications
IHC-P, IF, WB, FACS
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the recombinant EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant EpCAM antibody is available for research use only.
Storage Conditions
Store the recombinant EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Product Datasheet
https://www.nsjbio.com/tds-pdf/recombinant-epcam-antibody-rmoc-31-v3464
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Location
Cell surface, cytoplasmic
Image Legend
Immunofluorescence testing of human MCF7 cells with recombinant EpCAM antibody (green, clone rMOC-31) and RedDot nuclear stain (red) .
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