Recombinant EpCAM Antibody

Binding epitope of this antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59 of Ep-CAM. EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM) . It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Flow cytometry: 1-2ug/million cells

UniProt

P16422

Host

Mouse

Reactivity

Human

Immunogen

Neuraminidase treated GLS-1 human small cell lung carcinoma cells were used as the immunogen for the recombinant EpCAM antibody. Binding epitope of the rMOC-31 antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RMOC-31

Type

Recombinant

Applications

IHC-P, IF, WB, FACS

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant EpCAM antibody is available for research use only.

Storage Conditions

Store the recombinant EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant EpCAM antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.