Ep-CAM Antibody
EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM) . It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Product Specifications
CAS Number
9007-83-4
Specifications
UniProt
P16422
Host
Mouse
Reactivity
Human, Dog, Cat
Immunogen
A human partial recombinant protein corresponding to amino acids 100-224 (extracellular domain) was used as the immunogen for the Ep-CAM antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
EGP40/1798
Applications
ELISA, FACS, IF, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Ep-CAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Ep-CAM antibody is available for research use only.
Storage Conditions
Store the Ep-CAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Titering of the Ep-CAM antibody may be required for optimal performance.
Location
Cell surface, cytoplasmic
Image Legend
Immunofluorescent staining of human MCF-7 cells with Ep-CAM antibody (green, clone EGP40/1798) and Reddot nuclear stain (red) .
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