TOP2A Antibody / Topoisomerase II alpha

This mAb recognizes an ~170kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P11388

Host

Mouse

Reactivity

Human

Immunogen

Amino acids 1352-1493 from the human protein were used as the immunogen for this TOP2A antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

TOP2A/1362

Applications

WB, IF, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the TOP2A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This TOP2A antibody is available for research use only.

Storage Conditions

Store the TOP2A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TOP2A antibody to be titered up or down for optimal performance.

Location

Nuclear

Image Legend

IHC testing of FFPE human tonsil with TOP2A antibody (clone TOP2A/1362) . Required HIER: boil sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.