Retinoblastoma Antibody
Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P06400
Host
Mouse
Reactivity
Human
Immunogen
A human recombinant partial protein was used as the immunogen for this Retinoblastoma antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
SPM353
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
The Retinoblastoma antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.
Limitations
This Retinoblastoma antibody is available for research use only.
Storage Conditions
The Retinoblastoma antibody (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Titering of the Retinoblastoma antibody may be required for optimal performance.
Location
Nuclear
Image Legend
Frequently Asked Questions
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