Retinoblastoma Antibody
Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P06400
Host
Mouse
Reactivity
Human
Immunogen
A human recombinant partial protein was used as the immunogen for this Retinoblastoma antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
SPM353
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
The Retinoblastoma antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.
Limitations
This Retinoblastoma antibody is available for research use only.
Storage Conditions
The Retinoblastoma antibody (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Titering of the Retinoblastoma antibody may be required for optimal performance.
Location
Nuclear
Image Legend
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
PI 3 Kinase catalytic subunit gamma (PIK3CG) (NM_002649) Human Recombinant Protein
AIF1 / Iba1 (Microglia Marker) (AIF1/2493), CF594 conjugate, 0.1mg/mL
![MEIS1 Mouse Monoclonal Antibody [Clone ID: OTI2A3]](https://cdn.genprice.uk/products/937/68dd18ba061270361e90865e/imageurl/cf809619.webp)
