Elastase 3B Antibody / CELA3B
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA: 2-4 µg/mL (order BSA/azide-free format), Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P08861
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
A partial recombinant protein (aa 82-238) was used as the immunogen for the Elastase 3B antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
CELA3B/1257
Applications
ELISA, WB, IHC-P
Purity
Protein G
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Elastase 3B antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Elastase 3B antibody is available for research use only.
Storage Conditions
Store the Elastase 3B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Product Datasheet
https://www.nsjbio.com/tds-pdf/elastase-3b-antibody-cela3b-cela3b1257-v3436
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Elastase 3B antibody should be determined by the researcher.1. FFPE staining requires
Location
Cytoplasmic
Image Legend
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