S100A4 Antibody / FSP1
S100A4/FSP1 (Fibroblast specific protein 1) belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including p53, p63, non-muscle myosin IIA, a6b4 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P26447
Host
Mouse
Reactivity
Human, Mouse
Immunogen
Human recombinant S100A4 protein was used as the immunogen for this S100A4 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
S100A4/1482
Applications
FACS, IF, WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the S100A4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This S100A4 antibody is available for research use only.
Storage Conditions
Store the S100A4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
The optimal dilution of the S100A4 antibody for each application should be determined by the researcher.
Location
Cytoplasmic, nuclear
Image Legend
Western blot testing of human 1) HeLa, 2) A549 and 3) A431 cell lysate with S100A4 antibody (clone S100A4/1482) . Predicted molecular weight ~12 kDa.
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