FSP1 Antibody / S100A4

S100A4/FSP1 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4/FSP1 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, 4 integrin, and liprin b1. It is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 5ul/million cells, Western blot: 2-4 µg/mL, Immunofluorescence: 2-4 µg/mL at RT, Immunohistochemistry (FFPE) : 2-4 µg/mL at RT

UniProt

P26447

Host

Mouse

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 1-200 from the human protein was used as the immunogen for the FSP1 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

S100A4/1481

Applications

FACS, WB, IF, IHC-P

Purity

Protein G affinity chromatography

Format

Biotin Conjugate

Buffer

0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the FSP1 antibody at 2-8oC (up to one month) or aliquot and store at -20oC (longer term).

Limitations

This FSP1 antibody is available for research use only.

Storage Conditions

Store the FSP1 antibody at 2-8°C (up to one month) or aliquot and store at -20°C (longer term) .

Formulation

0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the FSP1 antibody should be determined by the researcher.

Location

Cytoplasmic and nuclear

Image Legend

IHC testing of FFPE human placenta with biotinylated FSP1 antibody (clone S100A4/1481) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.