FSP1 Antibody / S100A4

S100A4/FSP1 (Fibroblast specific protein 1) belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. FSP1 exerts its function via direct interaction with a number of proteins including p53, p63, non-muscle myosin IIA, a6b4 integrin, and liprin b1. FSP1 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P26447

Host

Mouse

Reactivity

Human, Mouse

Immunogen

A recombinant human partial protein (amino acids 1-200) was used as the immunogen for the recombinant FSP1 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

S100A4/1481

Applications

FACS, IF, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the FSP1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This FSP1 antibody is available for research use only.

Storage Conditions

Store the FSP1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The optimal dilution of the FSP1 antibody for each application should be determined by the researcher.

Location

Cytoplasmic, nuclear

Image Legend

IHC testing of FFPE human placenta with FSP1 antibody (clone S100A4/1481) . Required HIER: steam sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling.