TSH Receptor Antibody (beta chain)

Thyroid-stimulating hormone (TSH, also known as thyrotropin) is a glycoprotein involved in the control of thyroid structure and metabolism, which stimulates the release of the thyroid hormones. TSH is regulated by thyroid hormone (T3) and various retinoid compounds. TSH binds to the thyroid-stimulating hormone receptor (TSHR), which is cleaved into two subunits, A and B, and plays a major role in regulating thyroid function. The third cytoplasmic loop of TSHR has been identified as critical for its role in regulating inositol phosphate and cAMP formation. In Graves disease, an autoimmune disorder, TSHR is activated by autoantibodies, which may be stimulated by the cleavage of the A and B subunits.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P16473

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human TSHR beta chain protein was used as the immunogen for this TSH Receptor antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

SPM222

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the TSH Receptor antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This TSH Receptor antibody is available for research use only.

Storage Conditions

Store the TSH Receptor antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TSH Receptor antibody to be titered up or down for optimal performance.

Location

Cytoplasmic, cell surface

Image Legend

IHC testing of FFPE human thyroid carcinoma with TSH Receptor antibody (clone SPM222) . Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 8, for 10-20 min followed by cooling at RT for 20 min.