CFTR Antibody

Cystic fibrosis transmembrane conductance regulator (CFTR) is involved in the transport of chloride ions. May regulate bicarbonate secretion and salvage in epithelial cells by regulating the SLC4A7 transporter. Can inhibit the chloride channel activity of ANO1. Plays a role in the chloride and bicarbonate homeostasis during sperm epididymal maturation and capacitation. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/10^6 cells, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P13569

Host

Mouse

Reactivity

Human

Immunogen

A partial recombinant protein corresponding to amino acids 258-385 from the human protein was used as the immunogen for this CFTR antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

CFTR/1643

Applications

FACS, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the CFTR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CFTR antibody is available for research use only.

Storage Conditions

Store the CFTR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CFTR antibody to be titered up or down for optimal performance.

Location

Cell surface, cytoplasmic

Image Legend

IHC testing of FFPE human pancreas with CFTR antibody (clone CFTR/1643) . HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9 for 10-20 min followed by cooling at RT for 20 min.