TSHR Antibody alpha chain

Thyroid-stimulating hormone (TSH, also known as thyrotropin) is a glycoprotein involved in the control of thyroid structure and metabolism, which stimulates the release of the thyroid hormones. TSH is regulated by thyroid hormone (T3) and various retinoid compounds. TSH binds to the thyroid-stimulating hormone receptor (TSHR), which is cleaved into two subunits, A and B, and plays a major role in regulating thyroid function. The third cytoplasmic loop of TSHR has been identified as critical for its role in regulating inositol phosphate and cAMP formation. In Graves disease, an autoimmune disorder, TSHR is activated by autoantibodies, which may be stimulated by the cleavage of the A and B subunits.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: 2-4 µg/mL (order BSA/azide-free format), Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P16473

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human TSH Receptor alpha chain protein was used as the immunogen for this TSHR antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

TSHRA/1402

Applications

ELISA, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the TSHR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This TSHR antibody is available for research use only.

Storage Conditions

Store the TSHR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TSHR antibody to be titered up or down for optimal performance.

Location

Cytoplasmic, cell surface

Image Legend

IHC testing of FFPE human thyroid carcinoma with TSHR antibody (clone TSHRA/1402) . Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, or 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.