VWF Antibody

Von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi's sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P04275

Host

Mouse

Reactivity

Human

Immunogen

Amino acids 1815-1939 from the human protein were used as the immunogen for this vWF antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

VWF/1465

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the vWF antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This vWF antibody is available for research use only.

Storage Conditions

Store the vWF antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The optimal dilution of the vWF antibody for each application should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Western blot testing of 1) partial recombinant protein and 2) human lung lysate with vWF antibody (clone VWF/1465) . Predicted molecular weight ~250 kDa.