CDX2 Antibody

The intestine-specific transcription factors CDX1 and CDX2 are important for directing intestinal development, differentiation, proliferation and maintenance of the intestinal phenotype. CDX2 protein expression has been seen in GI carcinomas. Anti-CDX2 has been useful to establish GI origin of metastatic adenocarcinomas and carcinoids and is especially useful to distinguish metastatic colorectal adenocarcinoma from lung adenocarcinoma. However, mucinous carcinomas of the ovary also express CDX2 protein. It limits the usefulness of this marker in the distinction of metastatic colorectal adenocarcinoma from mucinous carcinoma of the ovary.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: 2-4 µg/mL (order BSA/azide-free format), Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q99626

Host

Mouse

Reactivity

Human

Immunogen

Amino acids 150-249 from the human protein were used as the immunogen for this CDX2 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

CDX2/1690

Applications

ELISA, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the CDX2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CDX2 antibody is available for research use only.

Storage Conditions

Store the CDX2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CDX2 antibody to be titered up or down for optimal performance.

Location

Nuclear

Image Legend

IHC testing of FFPE human colon carcinoma with CDX2 antibody (clone CDX2/1690) . Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min.