PAX8 Antibody

PAX8 (and PAX2) is one of the important regulators of urogenital system morphogenesis. They play a role in the specification of the first renal cells of the embryo and remain essential players throughout development. [Wiki]

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q06710

Host

Mouse

Reactivity

Human

Immunogen

A human recombinant fragment (aa 60-261) was used as the immunogen for the PAX8 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

PAX8/1492

Applications

FACS, IF, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the PAX8 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This PAX8 antibody is available for research use only.

Storage Conditions

Store the PAX8 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

The stated application concentrations are suggested starting points. Titration of the PAX8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear, cytoplasmic

Image Legend

IHC testing of FFPE human thyroid carcinoma with PAX8 antibody (clone PAX8/1492) . Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 minutes.