NOX4 Antibody / NADPH Oxidase 4
The superoxide-generating NADPH oxidase includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with the flavocytochrome, cytochrome b558, to form the active enzyme complex. The p22 and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. NOX4 is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. It is also expressed in fetal tissues, placenta, glioblastoma and vascular cells.
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA (order BSA/sodium azide-free format for coating), Western blot: 0.5-1 µg/mL
UniProt
Q9NPH5
Host
Mouse
Reactivity
Human
Immunogen
Human recombinant protein was used as the immunogen for this NOX4 antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
NOX4/1245
Applications
ELISA, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the NOX4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This NOX4 antibody is available for research use only.
Storage Conditions
Store the NOX4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Titering of the NOX4 antibody may be required for optimal performance.
Location
Cytoplasmic and cell surface
Image Legend
SDS-PAGE Analysis of Purified, BSA-Free NOX4 Antibody (clone NOX4/1245) . Confirmation of Integrity and Purity of the Antibody.
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