Thrombomodulin Antibody / CD141

It recognizes a protein of 75kDa, identified as Thrombomodulin. Thrombomodulin is a transmembrane glycoprotein with natural anticoagulant properties. It is normally expressed by a restricted number of cells, such as endothelial and mesothelial cells. In addition, synovial lining and syncytio-trophoblasts of placenta also express thrombomodulin. This protein is present in almost all of benign vascular tumors and majority of malignant vascular tumors (Kaposi s sarcoma, angiosarcoma, and epithelioid hemangioendothelioma) . Hence, anti-thrombomodulin serves as a sensitive marker for lymphatic endothelial cells and their tumors. Recently, thrombomodulin antibody has been used for mesothelial cells and malignant mesotheliomas.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: 2-4 µg/mL (order BSA/azide-free format), Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P07204

Host

Mouse

Reactivity

Human

Immunogen

Human recombinant partial protein corresponding to amino acids 69-194 was used as the immunogen for this Thrombomodulin antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

THBD/1591

Applications

ELISA, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the Thrombomodulin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Thrombomodulin antibody is available for research use only.

Storage Conditions

Store the Thrombomodulin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Thrombomodulin antibody to be titered up or down for optimal performance.

Location

Cell surface, cytoplasmic

Image Legend

IHC testing of FFPE human bladder carcinoma with Thrombomodulin antibody (clone THBD/1591) . HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.