Spectrin beta III Antibody / SPTBN2

Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Spectrin alpha and spectrin beta are present in erythrocytes, whereas spectrin alpha II (fodrin a) and spectrin beta I (fodrin b) are present in other somatic cells. SPTBN2/Spectrin beta III is highly homologous to both spectrin beta I and spectrin beta II. SPTBN2 is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: 2-4 µg/mL (order BSA/azide-free format), Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL, Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

UniProt

O15020

Host

Mouse

Reactivity

Human

Immunogen

Human recombinant partial protein corresponding to amino acids 356-475 was used as the immunogen for this Spectrin beta III antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

SPTBN2/1582

Applications

ELISA, FACS, IF, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Spectrin beta III antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Spectrin beta III antibody is available for research use only.

Storage Conditions

Store the Spectrin beta III antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Spectrin beta III antibody to be titered up or down for optimal performance.

Location

Cell surface, cytoplasm

Image Legend

IHC testing of FFPE human pancreas with Spectrin beta III antibody (clone SPTBN2/1582) . Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.