Podoplanin Antibody

Podoplanin (PDPN) is a muco-protein which localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. It is selectively expressed in lymphatic endothelium as well as lymphoangiomas, Kaposi sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. Recent studies have also shown podoplanin to be a highly sensitive and relatively specific marker for epithelioid mesothelioma. Therefore, it can be used in a panel to distinguish mesotheliomas or mesothelial cells from pulmonary carcinomas.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order BSA/sodium azide-free format for coating), Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q86YL7

Host

Mouse

Reactivity

Human

Immunogen

A human recombinant partial protein corresponding to amino acids 24-126 was used as the immunogen for the Podoplanin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

PDPN/1433

Applications

ELISA, IF, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Podoplanin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Podoplanin antibody is available for research use only.

Storage Conditions

Store the Podoplanin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Podoplanin antibody should be determined by the researcher.

Location

Cell surface, cytoplasmic

Image Legend

Analysis of HuProt (TM) microarray containing more than 19,000 full-length human proteins using Podoplanin antibody (clone PDPN/1433) . These results demonstrate the foremost specificity of the PDPN/1433 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.