NSE Antibody / Neuron Specific Enolase
Recognizes a protein of about 50kDa, which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.2-0.4 µg/mL for 30 min at RT
UniProt
P09104
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids 416-433 of human Neuron Specific Enolase were used as the immunogen for this NSE antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
ENO2/1375
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the NSE antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This NSE antibody is available for research use only.
Storage Conditions
Store the NSE antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Location
Cytoplasmic
Image Legend
IHC testing of FFPE human cerebellum with NSE antibody (clone ENO2/1375) . Required HIER: boil sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
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