NSE Antibody / Neuron Specific Enolase

Recognizes a protein of about 50kDa, which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.2-0.4 µg/mL for 30 min at RT

UniProt

P09104

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 416-433 of human Neuron Specific Enolase were used as the immunogen for this NSE antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

ENO2/1375

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the NSE antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This NSE antibody is available for research use only.

Storage Conditions

Store the NSE antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NSE antibody to be titered up or down for optimal performance.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human cerebellum with NSE antibody (clone ENO2/1375) . Required HIER: boil sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.