Plakophilin 1 Antibody

Recognizes a protein of 75kDa, identified as Plakophilin 1 (PKP1) . Plakophilins 1, 2, 3 and 4 (PKP1-4) influence development and participate in linking cadherins to cytoskeletal intermediate filaments. PKP1-4 contain arm-repeat (armadillo) domains and localize to nuclei and cell desmosomes (cell-cell junctions found in suprabasal layers of stratifying epithelia that undergo mechanical stress) . PKP2 is important for desmosome assembly and is an essential morphogenic factor and architectural component of the heart.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 0.5-1 µg/mL

UniProt

Q13835

Host

Mouse

Reactivity

Human, Mouse

Immunogen

A N-terminal human recombinant protein fragment was used as the immunogen for the Plakophilin 1 antibody. Its epitope maps to between amino acids 1-27 of human PKP1.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

10B2

Applications

FACS, IF, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Plakophilin 1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Plakophilin 1 antibody is available for research use only.

Storage Conditions

Store the Plakophilin 1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Plakophilin 1 antibody should be determined by the researcher.

Location

Cell surface, nuclear

Image Legend

Western blot testing of human 1) U87, 2) HeLa and 3) A431 cell lysate with Plakophilin 1 antibody (clone 10B2) . Expected molecular weight: 75-83 kDa.